Live births from frozen human semen stored for 40 years

Effect of selection for fertility of frozen-thawed semen in chickens on the fertility of fresh and stored semen.

Studies were conducted using 68 males from the seventh generation of a line of meat-type breeder chickens selected for increased duration of fertility of frozen-thawed semen and 69 males of the randomly selected control line. These chickens were used to determine the presence or absence of differences between the selected and control lines with respect to fertility and spermatozoal motility whe...

Live Pups from Evaporatively Dried Mouse Sperm Stored at Ambient Temperature for up to 2 Years

The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples sto...

Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report

Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to...

Normal pregnancies and live births after autograft of frozen-thawed hemi-ovaries into ewes.

OBJECTIVE To evaluate long-term outcome of autotransplantation of cryopreserved hemi-ovaries into ewes. DESIGN Animal study. SETTING University fertility center, Hospices Civils de Lyon; and Ecole Nationale Vétérinaire de Lyon. PATIENT(S) Grivette ewes. INTERVENTION(S) Six hemi-ovaries from 6 ewes aged 6 to 12 months were frozen with a slow cooling protocol using 2 M of dimethyl sulfoxi...

Live Without Calcineurin Inhibitors : 40 Years Later , Can ' t