LIQUID NITROGEN FREEZING IN MICROBIOLOGICAL ASSAY SYSTEMS II
نویسندگان
چکیده
منابع مشابه
Liquid Nitrogen Freezing in Microbiological Assay Systems. Ii. Preservation of Sarcina Lutea for Antibiotic Assays.
The storage of Lactobacillus leichmannii in liquid nitrogen for direct use in the vitamin B12 assay was described by Sokolski, Stap)ert, and Ferrer (Appl. Microbiol. 12:327, 1964). This note describes a different ap)proach on treatment of data for stability of a test organism. A stable susl)ension of Sarcina lutea ATCC 9341, a bacterium used in many antibiotic assays, was obtained with storage ...
متن کاملLiquid Nitrogen Freezing in Microbiological Assay Systems. I. Preservation of Lactobacillus Leichmannii for Direct Use in the Vitamin B12 Assay.
Suspensions of Lactobacillus leichmannii were stored in liquid nitrogen and were used as direct inocula in vitamin B(12) assays. Complete recovery of viable cells was obtained when the suspensions in basal B(12) medium were rapidly frozen by direct immersion into liquid nitrogen and rapidly thawed by agitating the suspensions in a water bath at 40 C. Greater than 90% destruction occurred when t...
متن کاملPreservation of microbiological assay organisms by direct freezing.
'rhe effects of freezinig on bacterial cultures have been investigated by numerous workers. The most commonly used method of preserving microorganisms is lvophilization (drying from the frozen state), but this is a drastic procedure for in most instances comparatively few organisms (2 to 3 per cent) survive the freeze drying process. Cultures preserved in this way have remained viable for as lo...
متن کاملFreezing and Storing Ditylenchus dipsaci in Liquid Nitrogen.
After 18 months of storage at -150 C, some larvae of Ditylenchus dipsaci, which had been treated in a 7.5% solution of dimethyl sulphoxide and cooled to -25 C before storage, were still viable on thawing. Some survivors penetrated and developed normally in stems of alfalfa seedlings. Tests showed that active larvae could be frozen directly, thus eliminating the need to use the quiescent stage o...
متن کاملMorphology of heart valves preserved by liquid nitrogen freezing.
As a preliminary to establishing a frozen valve bank for replacement surgery, the possible effects of the proposed freezing and thawing procedure on tissue structure were assessed in 16 human pulmonary valves removed from cadavers at necropsy and nine dog valves obtained fresh. The valves were frozen and stored in liquid nitrogen for intervals ranging from 23 to 380 days. Blocks of tissue cut f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.88.2.532-533.1964