Liquid chromatographytandem mass spectrometry methods for determination of stanozolol and l6β-hydroxy-stanozolol in animal urine

Abstract Introduction Because of the activities and effects they induce, hormones are prohibited for use anabolic purposes in farm animals intended slaughter, which is regulated European Union by relevant legal provisions. Therefore, there an obligation to monitor residues food animal origin ensure consumer safety. A hormone banned but used formerly fattening cattle, stanozolol, its metabolite 16β-OH-stanozolol synthetic compounds that belong a large group steroid hormones. This study investigates these urine. Material Methods From 2006–2022, 2,995 livestock urine samples were tested stanozolol Poland as part National Residue Monitoring Programme. liquid chromatography–tandem mass spectrometry method determine was developed validated according required criteria. Urine sample analysis based on enzymatic hydrolysis potentially present it free form, extraction them from with mixture n-hexane butyl alcohol, purification extract NH2 amine column finally, instrumental detection. Results The apparent recovery precision parameters line established criteria, while decision limits CCα detection capabilities CCβ lower than recommended concentration analytical set at 2 μg L −1 (valid until December 15, 2022; currently 0.5 ). Conclusion All examined compliant evaluation