Liposome-mediated gene transfer to lung isografts
نویسندگان
چکیده
منابع مشابه
Adenoviral-mediated gene delivery to liver isografts: improved model of ex vivo gene transfer.
PREVIOUS studies using adenoviral vectors in experimental liver transplant settings have demonstrated in vivo infectivity by perfusing grafts with viral vectors. 1•2 However. these techniques required high viral titers (1 to 5 X 1010 plaque-forming units [pfu)) to induce effective viral infection. In this study, we developed an improved gene delivery method that allows near maximal infectivity ...
متن کاملLiposome-mediated gene transfer into human vascular smooth muscle cells.
BACKGROUND Complexing recombinant DNA with cationic liposomes is a convenient means of introducing foreign genes into cells (lipofection) and could potentially form the basis for genetically modifying diseased blood vessels in patients. The mechanism of lipofection is incompletely understood, but it is recognized that the degree of successful gene transfer is highly dependent on cell type. To d...
متن کاملLiposome mediated gene transfer into the rat oesophagus.
BACKGROUND Cancer of the oesophagus has so far eluded every attempt at pharmacological treatment. The recent advent of somatic gene therapy offers a new therapeutic approach to malignant tumours. AIM To investigate whether and how gene transfer into the oesophagus can be achieved. METHODS A LacZ reporter gene was used as marker and transferred into the oesophagus of rats using cationic lipo...
متن کاملCationic liposome-mediated gene delivery in vivo.
Several improvements have been made in liposomal delivery, thus making this technology potentially useful for treatment of certain diseases in the clinic. Success in non-viral delivery is complicated and requires optimization of several components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, ...
متن کاملCftr Gene Transfer to Primate Lung
We have evaluated the biological efficacy of El-deleted adenoviruses in baboons for lung-directed gene therapy of cystic fibrosis (CF). T h e experimental design attempted to simulate a phase I clinical trial with animals receiving a single dose of virus to an isolated pulmonary segment. A total of 14 animals divided into four groups, each of which received escalating doses of virus, were used....
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ژورنال
عنوان ژورنال: The Journal of Thoracic and Cardiovascular Surgery
سال: 1997
ISSN: 0022-5223
DOI: 10.1016/s0022-5223(97)70082-5