Lipid biomarkerek HPLC-HRMS vizsgálata történeti embertani minták tbc diagnosztikája során

Tuberculosis (TB) is not only an infectious disease but one of the top 10 causes death, spreading mainly with aerosol transmission and accompanying history humankind for several millennia. TB caused in humans animals by members Mycobacterium tuberculosis complex. For better understanding evolutional background its causative agent, involvement palaeopathological investigations surpassingly important. Traditionally, palaeopathology using a broad variety markers, which are observable macroscopic investigations. These markers related to different extra-pulmonary forms TB. However, these manifestations develop few cases. Moreover, many pathognomonic Therefore, avoid underestimation prevalence paleopathological studies, diagnosis on archaeological material requires multidisciplinary approach.
 estimation incidence past populations, array specific biomarkers can be brought into play confirm initial, macromorphology-based skeletal diagnoses, namely aDNA lipid biomarkers. The three biomarker groups, involved such mycolic acids (MAs), mycocerosic (MCs) C27 mycolipenic acid (ML). unique lipids located lipid-rich mycobacterial cell wall. application lipid-based diagnostic approach has been proved robust reliable through examples. A analytical methods have employed profiling. Fluorescence HPLC well-developed analysis phthiocerols. Furthermore, isolated case direct mass spectrometric detection M. free also introduced.
 Our aim was establish lipid-biomarker-based HPLC-MS method historical human samples, as this instrumentation available laboratories, potential quick sensitive at same time affordable measurement protocol. We successfully developed optimised method, capable separate detect MAs MCs. we sample pre-treatment process. started build MA MC profile library, serve comparison diagnose TB.
 verification new reliability analysis, palaeopathologists usually use well-documented collections skeletons mummies from pre-antibiotic era. purpose, chose test our Vác Mummy Collection. In four out six mummified individuals, profiles characteristic MCs were recorded. HPLC-ESI-MS MCs, opens avenue ancient disease, encompassing both tuberculosis, leprosy joint