Lipase Production by Yarrowia lipolytica in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate

The production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed through the application lipase. However, high cost associated with enzyme make process unfeasible thus alternative substrates should investigated solve these problems. This research aimed produce lipase by Yarrowia lipolytica IMUFRJ50682 in solid-state fermentation using by-products food processing industry (andiroba oil cake soybean meal) verify potential initial hydrolysis further a suitable process. A screening was carried out for analysis andiroba meal combinations different proportions (0:100 100:0, respectively) at 48 h Afterward, solid matrix composed supplemented soy Tween 80 improve activity. enzymatic extract characterized relation protein profile electrophoresis. Finally, biocatalyst produced were applied evaluate an study. Maximum lipolytic activity (63.7 U·g−1) achieved (50:50) after 24 fermentation. Soybean 1.5% (0.001%) emulsion provided increase 1.5-fold (82.52 electrophoretic demonstrated band between 37 40 kDa that may 75 referring α subunit β-conglycinin present meal. After h, showed higher degree (DH) (71.0%) than (61.5%). stable during storage room temperature 7 months. lipases Amazon fruit by-product viable as well industrially suited