LINC00460 mediates HMGA2 expression through binding to miRNA-143-5p competitively in gastric carcinoma

Background: Compelling evidence has manifested a strong association between aberrant expression of long noncoding RNAs (lncRNAs) and gastric carcinoma (GC) development. Nonetheless, biological impacts differentially expressed lncRNAs (DElncRNAs) on GC are not scrutinized. Methods: Bioinformatics methods were employed for differential analysis target gene prediction. MTT, colony formation, Transwell implemented cell proliferation, migration, invasion assessment. Western blot was to test the protein level. The binding genes tested with dual-luciferase RNA immunoprecipitation (RIP) approaches. Results: Noticeably high level LINC00460 observed in tissues cells. silencing constrained FISH nuclear-cytoplasmic separation assays confirmed main presentation cytoplasm. predicted that had sites miRNA-143-5p, which upregulated GC. Dual luciferase RIP experiments also relationship. Concurrent LINC00460s miRNA-133-5p rescued repressive influence sh-LINC004600 invasion. HMGA2 be downstream their relationship validated via dual assays. Silencing invasion, migration. modulated thereby affecting migration Conclusion: These findings could regulate sponging miRNA-143-5p facilitate provides deeper understanding development