Limulus Amoebocyte Lysate (LAL) Test - An Alternative Method for Detection of Bacterial Endotoxins
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چکیده
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Endotoxins and Their Detection with the Limulus Amebocyte Lysate Test
In contrast to the detailed descriptions of techniques provided by Larsson, a chapter on the measurement and analysis of acetylcholine written by Israel Hanin takes the form of a brief but comprehensive survey of all of the methods for the quantitation of acetylcholine introduced between 1933 and 1981. Each method presented is referenced and briefly critiqued. This is followed by an article by ...
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Twenty-eight antibiotics were tested with the Limulus amoebocyte lysate assay to determine their non-inhibitory concentrations (NICs). The Limulus amoebocyte lysate assay was found to be a valid test for most of the antibiotics tested; the NICs were found to be greater than the minimum valid test concentrations. Borderline results were obtained with cefamandole nafate and neomycin sulfate. Poly...
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The chromogenic Limulus amoebocyte lysate test effectively detected 66 (100%) culture-proven gram-negative peritonitis cases among 185 continuous ambulatory peritoneal dialysis patients with clinical evidence of infectious peritonitis.
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A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.
متن کاملBiochemical principle of Limulus test for detecting bacterial endotoxins
A hemocyte lysate from horseshoe crab (Limulus) produced a gel, when exposed to Gram-negative bacterial endotoxins, lipopolysaccharides (LPS). This gelation reaction of the lysate, so-called Limulus test, has been widely employed as a simple and very sensitive assay method for endotoxins. Recent biochemical studies on the principle of Limulus test indicate that the hemocytes contain several ser...
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ژورنال
عنوان ژورنال: Acta Veterinaria Brno
سال: 2001
ISSN: 0001-7213,1801-7576
DOI: 10.2754/avb200170030291