LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY
نویسندگان
چکیده
منابع مشابه
Limiting factors in single particle cryo electron tomography
Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within tomograms allow exploring their structures within the native context of the cell, avoiding the need for protein isolation and purification. T...
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Biological samples are very sensitive to beam irradiation because of induced changes to their structure by the incident electron. Due to the limited allowed dose, cryo-images of frozen-hydrated biological specimens are typically noisy and low in contrast. One way to improve the imaging of such samples is by using a phase plate to increase contrast and signal-to-noise ratio. The improved contras...
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ژورنال
عنوان ژورنال: Computational and Structural Biotechnology Journal
سال: 2012
ISSN: 2001-0370
DOI: 10.5936/csbj.201207002