Limitation of nutrients stimulates musty odor production by <i>Streptomyces</i> sp. isolated from a tropical environment

Musty odor production by actinomycetes is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB), which are synthesized enzymes encoded geoA tpc genes, respectively. Streptomyces spp. strain S10, was isolated from a water reservoir in Malaysia, has ability produce when cultivated basal salt (BS) solid medium, but no 2-MIB occurred during growth BS medium. Strain S10 could higher levels phosphate concentration limited 0.05 mg/L, with yield 17.53 ± 3.12 ✕ 105 ng/L, compared Interestingly, suddenly detected nitrate 1.0 1.4 0.11 ng/L. Therefore, it concluded that phosphate- nitrate-limiting conditions induce initial S10. Furthermore, positive amplicon PCR analysis. Draft genome sequence analysis showed one open reading frame (ORF) contained conserved motif synthase 95% identity coelicolor A3 (2). In case found ORF 23% known gene S. A3(2), lacked N-terminus.