LIMCH1 protein expression associated with lymph node metastasis in breast cancer

Background. Lymph node metastasis in breast cancer is not only a variant of progression, but also associated with the development hematogenous metastases. The LIMCH1 protein involved mesenchymal type migration due to activation non-muscle myosin IIA. objective study was investigate association different expression variants lymph metastasis. Material and Methods. retrospective included 53 patients invasive carcinoma no special type. group all molecular genetic types (luminal A, luminal B, HER2-positive triple negative). median age 55.4 ± 14 years. According TNM classification, had stage T1–3N0–3M0 cancer. did receive preoperative chemotherapy. tumor cells assessed using immunohistochemistry. Anti-LIMCH1Prestige (HPA004184, Sigma Aldrich, Germany) anti-LIMCH1-C-term (SAB2700402, antibodies were used. Results. metastases more frequent cells. This true for cytoplasmic detected by CH domain, submembrane or LIM regardless localization cell. phenomenon morphological heterogeneity most unfavorable factor worse metastasis-free survival combination presence domain. Conclusion. synchronous domain primary tissue.