Light-scattering spectroscopy differentiates fetal from adult nucleated red blood cells: may lead to noninvasive prenatal diagnosis
نویسندگان
چکیده
منابع مشابه
Isolation of fetal nucleated red blood cell from maternal blood using immunomagnetic beads for prenatal diagnosis.
The immunomagnetic beads method for isolation of fetal nucleated red blood cells (FNRBCs) from peripheral blood of 78 pregnant women for prenatal diagnosis was developed. The study subjects were classified into 8-10 and 11-14 weeks of gestation (n = 39 each). Peripheral blood cells were divided into two for the FNRBCs isolation using two protocols, one with anti-CD45 depletion followed by anti-...
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A model is presented to calculate the light-scatter properties of nucleated blood cells which are mimicked by two concentric spheres. The light-scatter characteristics were derived from the Rayleigh-Debye-Gans approximation and by simultaneous application of field averaging of the internal field and modification of the propagation constant inside the different cellular compartments. It is shown...
متن کاملNon-invasive Prenatal Diagnosis of Chromosomal Aneuploidies and Microdeletion Syndrome Using Fetal Nucleated Red Blood Cells Isolated by Nanostructure Microchips
Detection of detached fetal nucleated red blood cells (fNRBCs) in the maternal peripheral blood may serve as a prospective testing method competing with the cell-free DNA, in non-invasive prenatal testing (NIPT). Methods: Herein, we introduce a facile and effective lab-on-a-chip method of fNRBCs detection using a capture-releasing material that is composed of biotin-doped polypyrrole nanopartic...
متن کاملCulture of fetal cells from maternal blood for prenatal diagnosis.
The isolation and analysis of fetal cells from maternal blood would allow non-invasive prenatal genetic screening and diagnosis. Over the past decade, progress has been made towards this goal using various enrichment strategies and analysis by fluorescence in-situ hybridization with chromosome-specific probes and PCR. One method that is currently being explored involves culturing fetal cells. D...
متن کاملLight scattering by multiple red blood cells.
The interaction of light with multiple red blood cells was systematically investigated by the finite-difference time-domain method (FDTD). The simulations showed that the lateral multiple scattering between red blood cells is very weak and that the polarization has an almost insignificant influence on the distribution of the scattered light. The numerical results of the FDTD method were compare...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2009
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.34.001483