Ligation of Double-Stranded and Single-Stranded [Oligo(dT)] DNA by Vaccinia Virus DNA Ligase
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چکیده
منابع مشابه
Ligation of double-stranded and single-stranded [oligo(dT)] DNA by vaccinia virus DNA ligase.
Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed to either a poly(dA) or a poly(rA) backbone and, remarkably, free oligo(dT). Th...
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ژورنال
عنوان ژورنال: Virology
سال: 1996
ISSN: 0042-6822
DOI: 10.1006/viro.1996.0358