منابع مشابه
Shuffling Yeast Gene Expression Data
A new method to sort gene expression patterns into functional groups is presented. The method is based on a sorting algorithm using a non-local similarity score, which takes all other patterns in the dataset into account. The method is therefore very robust wih respect to noise. Using the expression data for yeast, we extract information about functional groups. Without prior knowledge of param...
متن کاملPredicting crossover generation in DNA shuffling.
We introduce a quantitative framework for assessing the generation of crossovers in DNA shuffling experiments. The approach uses free energy calculations and complete sequence information to model the annealing process. Statistics obtained for the annealing events then are combined with a reassembly algorithm to infer crossover allocation in the reassembled sequences. The fraction of reassemble...
متن کاملSynthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling.
Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv antibody library was constructed in single immunoglobulin framework to enable rapid affinity maturation by updated Kunkel's mutagenesis. The initial diversity was generated predominantly in the V(H) domain combined with only 36 V(L) domain vari...
متن کاملNext-Generation Library information service - 'Smart Library'
For a library to be able provide information services and fulfill its function as a knowledge convergence center capable of responding to various information demands, the development of next-generation information systems based on the latest information and communication technology is needed. The development of mobile information services using portable devices such smart phones and tablet PCs ...
متن کاملGeneration of families of construct variants using golden gate shuffling.
Current standard cloning methods based on the use of restriction enzymes and ligase are very versatile, but are not well suited for high-throughput cloning projects or for assembly of many DNA fragments from several parental plasmids in a single step. We have previously reported the development of an efficient cloning method based on the use of type IIs restriction enzymes and restriction-ligat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2014
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb1512s105