let-7 miRNAs Can Act through Notch to Regulate Human Gliogenesis

let-7 miRNAs Can Act through Notch to Regulate Human Gliogenesis

It is clear that neural differentiation from human pluripotent stem cells generates cells that are developmentally immature. Here, we show that the let-7 plays a functional role in the developmental decision making of human neural progenitors, controlling whether these cells make neurons or glia. Through gain- and loss-of-function studies on both tissue and pluripotent derived cells, our data s...

Circulating plant miRNAs can regulate human gene expression in vitro

While Brassica oleracea vegetables have been linked to cancer prevention, the exact mechanism remains unknown. Regulation of gene expression by cross-species microRNAs has been previously reported; however, its link to cancer suppression remains unexplored. In this study we address both issues. We confirm plant microRNAs in human blood in a large nutrigenomics study cohort and in a randomized d...

Up a Notch Instructing Gliogenesis

neurons and glia, can be purified and generate neurons, How Do Multipotent Neural Stem Cells glia, and myofibroblasts in vitro in response to known Generate Glia? instructive signals (Morrison et al., 1999). For instance, Our brains contain two principle classes of cells, neuBMPs promote neuronal differentiation, whereas Nrg1/ rons and glia, which are structurally and functionally GGF2 promotes...

Let-7 miRNAs target the lineage-specific transcription factor PLZF to regulate terminal NKT cell differentiation and effector function

Defining Transcriptional Regulatory Mechanisms for Primary let-7 miRNAs

The let-7 family of miRNAs have been shown to control developmental timing in organisms from C. elegans to humans; their function in several essential cell processes throughout development is also well conserved. Numerous studies have defined several steps of post-transcriptional regulation of let-7 production; from pri-miRNA through pre-miRNA, to the mature miRNA that targets endogenous mRNAs ...