Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay
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چکیده
منابع مشابه
Assaying Cellular Viability Using the Neutral Red Uptake Assay.
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investig...
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A neutral red assay involving Vero cells was used to quantitate the cytotoxic activity of verotoxins (VT) produced by Escherichia coli and to investigate changes in titer caused by altering the composition of the cell culture medium. Three variations on medium 199 were investigated: one involved supplementing the medium with 5% fetal bovine serum (FBS), a second was the use of serum-free (SF) m...
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Acute oral toxicity is currently being assessed by a suite of refinement test methods based on thetraditional LD50 lethality test and is, besides skin sensitisation, the only remaining animal test requiredunder REACH Annex VII. In view of assessing the use of alternatives for this health endpoint, EURL ECVAMconducted a study on the 3T3 Neutral Red Uptake cytotoxicity test method add...
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We report on progress towards a neutral atom imaging device that will be used for chemically sensitive surface microscopy and nanofabrication. Our novel technique for improving refractive power and correcting chromatic aberration in atom lenses is based on a fundamental paradigm shift from continuous-beam focusing to a pulsed, three-dimensional approach. Simulations of this system suggest that ...
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Neutral red (NR) is a dye that must be actively imported into the cell, and, therefore, the dye has been used for decades to selectively stain living cells. In addition, NR can also be incorporated into virus particles, although the mechanism behind this is poorly understood. Once encapsulated into the virion, NR, a light sensitive dye, can be photoactivated to inactivate the virus. The propose...
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ژورنال
عنوان ژورنال: ACS Applied Bio Materials
سال: 2020
ISSN: 2576-6422,2576-6422
DOI: 10.1021/acsabm.0c01082