LC–MS/MS simultaneous analysis of allopregnanolone, epiallopregnanolone, pregnanolone, dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate in human plasma
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چکیده
منابع مشابه
Metabolism of dehydroepiandrosterone sulfate and estrone-sulfate by human platelets.
The aim of the present research was to study the uptake of DHEAS, and to establish the intracrine capacity of human platelets to produce sex steroid hormones. The DHEAS transport was evaluated through the uptake of [(3)H]-DHEAS in the presence or absence of different substrates through the organic anion transporting polypeptide (OATP) family. The activity of sulfatase enzyme was evaluated, and ...
متن کاملDetermination of dehydroepiandrosterone in human blood plasma.
The first method for the determination of dehydroepiandrosterone (DHEA) in blood plasma was published by Migeon (1). To follow variations of DHEA in the blood within a rather short period, it seemed desirable to reduce not only the time necessary for one analysis, but also the volume of plasma needed for this procedure. Furthermore, by using the method of Migeon, our recoveries of added Na DHEA...
متن کاملSerum dehydroepiandrosterone, dehydroepiandrosterone sulfate, and pregnenolone sulfate concentrations in patients with hyperthyroidism and hypothyroidism.
BACKGROUND Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) have been suggested to have protective effects against cardiovascular disease, cancer, immune-modulated diseases, and aging. We examined serum concentrations of DHEA, DHEA-S, and pregnenolone sulfate (PREG-S) in patients with thyroid dysfunction. METHODS Steroids extracted with methanol from serum sample were...
متن کاملSimultaneous measurement of endogenous cortisol, cortisone, dehydroepiandrosterone, and dehydroepiandrosterone sulfate in nails by use of UPLC-MS-MS.
Steroid hormone concentrations are mostly determined by using different body fluids as matrices and applying immunoassay techniques. However, usability of these approaches may be restricted for several reasons, including ethical barriers to invasive sampling. Therefore, we developed an ultra-performance LC-MS-MS method for high-throughput determination of concentrations of cortisol, cortisone, ...
متن کاملHuman immunodeficiency virus infection and levels of Dehydroepiandrosterone sulfate in plasma among Indians.
The shift in cytokine profile during human immunodeficiency virus (HIV) disease progression is influenced by dehydroepiandrosterone sulfate (DHEAS) level. Radioimmunoassay was used to measure plasma DHEAS for 30 treatment-naïve HIV-infected and 30 uninfected individuals. There was a significant negative correlation of viral load with DHEAS level (P<0.05). Further studies of the use of DHEAS lev...
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ژورنال
عنوان ژورنال: Bioanalysis
سال: 2017
ISSN: 1757-6180,1757-6199
DOI: 10.4155/bio-2016-0262