LC-MS and GC-MS based metabolomics platform for cancer research

LC-MS and GC-MS based metabolomics platform for cancer research

We present here a LC-MS and GC-MS based analytical platform for the comprehensive analysis of cellular metabolites, including water-soluble metabolites, and waterinsoluble fatty acids, and phospholipids. The entire workflow consists of metabolic extraction, LC-MS and GC-MS runs, data analysis and interpretation. Standard operation procedures have been developed for the metabolite extraction fro...

Chemical Analysis of Pesticides Using GC/MS, GC/MS/MS, and LC/MS/MS

There are well over 500 registered pesticides worldwide for use in agricultural regions and new agrochemicals are introduced to the marketplace continuously. This chapter deals with the chemical analysis methods for the main pesticide chemical classes that are most frequently analyzed with gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS). GC amenable pesti...

Integrated Transcriptomics and Metabolomics Study of Retinoblastoma Using Agilent Microarrays and LC/MS/GC/MS Platforms

Organization of GC/MS and LC/MS metabolomics data into chemical libraries

BACKGROUND Metabolomics experiments involve generating and comparing small molecule (metabolite) profiles from complex mixture samples to identify those metabolites that are modulated in altered states (e.g., disease, drug treatment, toxin exposure). One non-targeted metabolomics approach attempts to identify and interrogate all small molecules in a sample using GC or LC separation followed by ...

Agilent Metabolomics Workflow Solutions Using LC/MS, GC/MS, and NMR

Agilent Technologies is an established leader in providing metabolomics solutions. Metabolomics refers to the identification and quantitation of metabolites, which are the small organic molecules that are used in biological processes within cells. These molecules are important indicators of biological function, and their study can provide unique insights into cell physiology, metabolism, and to...