LB1033 Thrombin contributes to atopic dermatitis pathogenesis and staphylococcus aureus skin colonization in children

Rationale: Adults with atopic dermatitis (AD) have an increased risk for thromboembolic events. Clotting factors been shown to impact Staphylococcus aureus (S. aureus) colonization. However, the interplay between pediatric AD, thrombin, and S. remain under investigated. Methods: To assess thrombin in AD we performed a plasma generation assay on samples from Mechanisms of Progression Atopic Dermatitis Asthma Children (MPAACH) cohort. We used SCoring (SCORAD) score, transepidermal water loss (TEWL), colonization as measures. determine whether contributes pathogenesis dabigatran, direct inhibitor, our established murine model AD. All mice underwent objective disease severity assessments TEWL evaluations. Analyses were using GraphPad Prism, version 9.0.0. Results: In children, found that total was associated moderate-to-severe (P-value=0.02) at lesional (P-value=0.001) never-lesional sites; peak (P-value=0.05) (P-value=0.01). Peak also skin by mouse mode inhibition attenuated development decreasing (P-value<0.001) (P-value<0.001). Conclusions: Our combined human findings highly demonstrate key role pathogenesis. Further, these data support children