Lattice filter for processing image data of three-dimensional protein nanocrystals

Lattice filter for processing image data of three-dimensional protein nanocrystals

When 300 kV cryo-EM images at Scherzer focus are acquired from ∼ 100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means o...

Image processing and lattice determination for three-dimensional nanocrystals.

Three-dimensional nanocrystals can be studied by electron diffraction using transmission cryo-electron microscopy. For molecular structure determination of proteins, such nanosized crystalline samples are out of reach for traditional single-crystal X-ray crystallography. For the study of materials that are not sensitive to the electron beam, software has been developed for determining the cryst...

Three-Dimensional Image of A Communicating Uterus

Recent advances in reproductive medicine have created a demand for more accurate and safe imaging modalities before assisted reproductive treatment(ART) (1). Gradually, the important role of three-dimensional (3D) ultrasonography in the diagnosis of uterine congenital anomalies has been proved (2-4).

Fundamentals of Three-dimensional Digital Image Processing

This book is a detailed description of the basics of 3-dimensional (3D) digital image processing, in particular the processing of images from techniques such as CT, MRI, and nuclear emission tomography. The book is based on 1980s research by the first author, Dr. Toriwaki, at Nagoya University and Chukyo University, Japan. The targeted audience is primarily graduate students and scientists in b...

Simulation of cake formation for nano-fibrous filter media using image processing