Large unit red cell cryopreservation with hydroxyethyl starch
نویسندگان
چکیده
منابع مشابه
Large unit red cell cryopreservation with hydroxyethyl starch.
Red blood cells can be preserved by freezing using either intraor extracellular agents. The most common intracellular agent, glycerol, is currently employed in general use and provides clinically acceptable red cells following post-thaw washing (13). Extracellular agents do not enter the cell during freezing. Therefore, red cells preserved with these agents may be administered without post-thaw...
متن کاملPost-thaw suspension of red cells cryopreserved with hydroxyethyl starch.
Post-thaw testing of large units of red cells frozen with 14% hydroxyethyl starch (HES) indicates 97-98s of the cells are recovered and about 85% are stable in saline (1). The quantity of supernatant hemoglobin present in such units at thaw is generally near 450-550 mgs. We have been unable to obtain consistent results which reveal a substantial improvement over these values, This quantity of f...
متن کاملHydroxyethyl starch in patients with trauma.
Editor—The Fluids in Resuscitation of Severe Trauma (FIRST) randomized trial conducted by James and colleagues compared hydroxyethyl starch (HES) 130/0.4 with 0.9% saline in the resuscitation of 115 patients with severe blunt or penetrating trauma at a single centre in South Africa. In blunt trauma, which accounts for 86–97% of all traumatic injuries in Europe, 3 no differences were found betwe...
متن کاملCryopreservation of dermal fibroblasts and keratinocytes in hydroxyethyl starch–based cryoprotectants
BACKGROUND Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is imperative. The aim of this study was to identify CPAs that minimize toxic effects and allow for the preservation of human fibroblasts and keratinocytes in suspension and in mon...
متن کاملRed cells preserved with 10% hydroxyethyl starch: effect of prefreeze washing.
Hydroxyethyl starch (HES) has shown promise as an extracellular cryoprotective agent for red blood cells. Since it is nontoxic and red cells are osmotically stable in its presence, a thawed unit could be administered without removing the protective agent. The most effective concentration of HES for red cell cryopreservation is 14% (2-4). Concentrations higher then this are extremely viscous whe...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 1976
ISSN: 0011-2240
DOI: 10.1016/0011-2240(76)90143-7