Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides
نویسندگان
چکیده
منابع مشابه
Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides.
We present a fast and simple protocol for large-scale preparation and purification of RNA oligonucleotides. RNA oligonucleotides are prepared by in vitro transcription with T7 RNA polymerase from linearized plasmid DNA templates constructed by PCR. In place of denaturing polyacrylamide gel electrophoresis (PAGE), size-exclusion chromatography is employed to purify the RNA oligonucleotide from t...
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ژورنال
عنوان ژورنال: RNA
سال: 2004
ISSN: 1355-8382
DOI: 10.1261/rna.5264804