Large-scale EM & Correlative Microscopy (Nanotomy & CLEM)
نویسندگان
چکیده
منابع مشابه
Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution electron microscopy. Others and we previously applied large scale EM to human skin pancreatic islets, tissue culture and whole zebrafish larvae(1-7). Here we describe a universally applicable method for tissue-scale scanning EM for unbiased detection of sub-cellular and molecular feature...
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Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate...
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The eukaryotic cell relies on complex, highly regulated, and functionally distinct membrane bound compartments that preserve a biochemical polarity necessary for proper cellular function. Understanding how the enzymes, proteins, and cytoskeletal components govern and maintain this biochemical segregation is therefore of paramount importance. The use of fluorescently tagged molecules to localize...
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Controlled Light Exposure Microscopy (CLEM): Photobleaching and Phototoxicity Reduced Photobleaching and phototoxicity are often the Achilles‘ heels of fluorescence live-cell imaging. Controlled Light Exposure Microscopy (CLEM) is a novel, simple imaging approach that reduces photobleaching and phototoxicity 2 to 10-fold without compromising image quality. The basic concept of CLEM is that ligh...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2016
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927616001847