Laccase from Sycamore Maple (Acer pseudoplatanus) Polymerizes Monolignols
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منابع مشابه
Laccase from Sycamore Maple (Acer pseudoplatanus) Polymerizes Monolignols.
Current understanding of the final oxidative steps leading to lignin deposition in trees and other higher plants is limited with respect to what enzymes are involved, where they are localized, how they are transported, and what factors regulate them. With the use of cell suspension cultures of sycamore maple (Acer pseudoplatanus), an in-depth study of laccase, one of the oxidative enzymes possi...
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Laccases (p-diphenol:O, oxidoreductase, EC 1.1 0.3.2) are m,embers of a highly conserved class of metalloenzymes, the ”blue” copper oxidases, which includes ascorbate oxidase and ceruloplasmin (Rydén and Hunt, 1993). First identified more than 100 years ago in extracts of sap from the Japanese lacquer tree (Rhus vernicifera), laccases have since been identified in fungi, insects, higher plants,...
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A laccase-type polyphenol oxidase is excreted by sycamore cells (Acer pseudoplatanus L.) cells. The enzyme has been purified by classical purification techniques. It is a blue copper protein of Mr 97 000, containing 45% carbohydrate and 0.24% copper. This protein consists of one single unit and the copper content corresponds to four copper atoms per protein molecule. The specific activity of th...
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Highly purified amyloplasts were isolated from cultured cells of sycamore (Acer pseudoplatanus L.). Incubation of amyloplasts with [gamma-(32)P]-ATP resulted in the labeling of more than ten polypeptides. Pulsechase experiments showed the reversibility of the process with some but not all of the polypeptides. The phosphorylation reaction of one polypeptide, M(r) 100, was shown to be calcium dep...
متن کاملEffect of gibberellic acid on germination capacity and emergence rate of Sycamore maple (Acer pseudoplatanus L.) seeds
Seedlots of sycamore maple (Acer pseudoplatanus L.) originating from three different provenance regions were collected in autumn 2012. The seed quality, namely vitality and germination rate, was assessed according to Czech conventions. There were four replications within each seedlot, i.e. twelve treatments altogether for each experimental variant. The effect of gibberellic acid on germination ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.99.3.1162