منابع مشابه
lac Operator nucleosomes. 2. lac Nucleosomes can change conformation to strengthen binding by lac repressor.
We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment. We describe here a quantitative method for determining the equilibrium binding affinities of repressor for these l...
متن کاملConstruction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.
Bacteriophage Mu d1 (lac Aprr) was used to obtain strains of Escherichia coli K-12 in which the lac genes are expressed from the promoter of pheA, the structural gene for the enzyme chorismate mutase P-prephenate-dehydratase. A derivative of bacteriophage lambda which carries the pheA-lac fusion was prepared; the method used is generally applicable for the construction, from Mu dl lysogens, of ...
متن کاملBl Lac Objects
SUMMARY: We present the radio detection of 18 BL Lac objects from our survey of over 575 deg 2 of sky. These 18 objects are located within 20 ′′ of the X-ray position, of which 11 have a measured red-shift. All candidates are radio emitters above ∼1 mJy and fall within the range of existing samples on the two colour, α ro vs α ox , diagram with a transitional population of three (3) evident. Tw...
متن کاملLac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.
As measured by a decreased rate of dissociation, lac repressor binds 10-times tighter to 5-bromodeoxyuridine-substituted lac operator than it does to normal lac operator. This result is obtained both in the absence and in the presence of isopropylthiogalactoside, an inducing ligand. These data are significant with regard to the mechanism of sequence-specific protein-DNA interaction, and also su...
متن کاملTransposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.
The power of recombinant DNA technology stems from the ability it provides to create novel DNA joints; this method uses enzymes to join unrelated DNA fragments in vitro. For example, two novel DNA joints are created when a gene is cloned in vitro onto a small plasmid vector. Transposition and illegitimate recombination also create novel DNA joints. Accordingly, when harnessed properly by geneti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Geographica Helvetica
سال: 1961
ISSN: 2194-8798
DOI: 10.5194/gh-16-161-1961