Laboratory Reporting of 25-Hydroxyvitamin D Results: Potential for Clinical Misinterpretation

Serum 25-hydroxyvitamin D immunoassays: recommendations for correct clinical interpretation.

assay on the Roche Modular analyzer. They used clinical samples that were almost free of 25hydroxyvitamin D2 (25-OH-D2) ( 10 nmol/L) as confirmed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and found a good overall agreement between the results obtained with the Roche assay and those measured with the DiaSorin RIA and by LC-MS/MS. Although they acknowledged that the Roche assay...

Measurement of 25-hydroxyvitamin D in the clinical laboratory: current procedures, performance characteristics and limitations.

In this review we describe procedures, performance characteristics and limitations of methods available for the measurement of 25-hydroxyvitamin (25OHD) since the year 2000. The two main types of methods are competitive immunoassay and those based on chromatographic separation followed by non-immunological direct detection (HPLC, LC-MS/MS). Lack of a reference standard for 25OHD has, until rece...

Inaccurate 25-hydroxyvitamin D results from a common immunoassay.

Serum 25-hydroxyvitamin D (25-OH-D) in obese adolescents.

BACKGROUND There is increasing evidence that vitamin D deficiency is common and has been associated with several non-bone related outcomes, including insulin resistance, type 2 diabetes and cardiovascular disease. The influences of gender, puberty, and adiposity on serum hydroxyvitamin D (25-OH-D) levels and the relationship between 25-OH-D and insulin resistance in obese children were studied....

Correlates of Circulating 25-Hydroxyvitamin D

Low vitamin D status is common globally and is associated with multiple disease outcomes. Understanding the correlates of vitamin D status will help guide clinical practice, research, and interpretation of studies. Correlates of circulating 25-hydroxyvitamin D (25(OH)D) concentrations measured in a single laboratory were examined in 4,723 cancer-free men and women from 10 cohorts participating ...