Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy.

Macrophages adopt a variety of phenotypes that are a reflection of the many functions they perform as part of the immune system. In particular, metabolism is a phenotypic trait that differs between classically activated, proinflammatory macrophages, and alternatively activated, prohealing macrophages. Inflammatory macrophages have a metabolism based on glycolysis while alternatively activated m...

متن کامل

Fluorescence lifetime imaging of free and protein-bound NADH.

We introduce a methodology, fluorescence lifetime imaging (FLIM), in which the contrast depends on the fluorescence lifetime at each point in a two-dimensional image and not on the local concentration and/or intensity of the fluorophore. We used FLIM to create lifetime images of NADH when free in solution and when bound to malate dehydrogenase. This represents a challenging case for lifetime im...

متن کامل

Tomographic fluorescence lifetime imaging

Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution lifetime images of thin tissue sections. This chapter concerns the in vivo time domain imaging of fluorescent contrast agents embedded in de...

متن کامل

Fluorescence lifetime imaging ophthalmoscopy

Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can...

متن کامل

Stroboscopic fluorescence lifetime imaging.

We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the relaxation rate of the fluorophores. The tech...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Annals of Biomedical Engineering

سال: 2018

ISSN: 0090-6964,1573-9686

DOI: 10.1007/s10439-018-2087-6