L2′ loop is critical for caspase-7 active site formation

L2' loop is critical for caspase-7 active site formation.

The active sites of caspases are composed of four mobile loops. A loop (L2) from one half of the dimer interacts with a loop (L2') from the other half of the dimer to bind substrate. In an inactive form, the two L2' loops form a cross-dimer hydrogen-bond network over the dimer interface. Although the L2' loop has been implicated as playing a central role in the formation of the active-site loop...

An arginine-aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation

Pseudouridine synthases introduce the most common RNA modification and likely use the same catalytic mechanism. Besides a catalytic aspartate residue, the contributions of other residues for catalysis of pseudouridine formation are poorly understood. Here, we have tested the role of a conserved basic residue in the active site for catalysis using the bacterial pseudouridine synthase TruB target...

Active Caspase-3 Is Stored within Secretory

Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics.

Product release is partially rate determining in the isomerization reaction catalyzed by Triosephosphate Isomerase, the conversion of dihydroxyacetone phosphate to D-glyceraldehyde 3-phosphate, probably because an active-site loop movement is necessary to free the product from confinement in the active-site. The timescale of the catalytic loop motion and of ligand release were studied using 19F...

7 L2 Gain 7.1 L2 Gain for Behavioral Models

7.1.1 L2 Gain for Continuous Time Signals Let S be a behavioral model of a continuous time system (i.e. S is a subset of the set L of all n-dimensional signals – locally square integrable functions v : [0,∞) 7→ R. Let Lw and Le be two fixed real matrices of dimension m-by-n and k-by-n respectively. For every v ∈ S, equalities w = Lwv and e = Lev define two signals w and e which can be viewed as...