L-Cysteine-Modified Transfersomes for Enhanced Epidermal Delivery of Podophyllotoxin

The purpose of this study was to evaluate L-cysteine-modified transfersomes as the topical carrier for enhanced epidermal delivery podophyllotoxin (POD). L-cysteine-deoxycholic acid (LC-DCA) conjugate synthesized via an amidation reaction. POD-loaded (POD-LCTs) were prepared a thin membrane dispersion method and characterized their particle size, zeta potential, morphology, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) in vitro release. Subsequently, skin permeation retention, fluorescence distribution skin, hematoxylin–eosin staining vivo irritation studied. POD-LCTs formed spherical shapes with size 172.5 ± 67.2 nm potential −31.3 6.7 mV. Compared POD-Ts, provided significantly lower drug penetration through porcine ear increased retention (p < 0.05). Meaningfully, unlike extensive (POD-Ts) throughout tissue, mainly located epidermis. Moreover, did not induce irritation. Therefore, might be promising POD.