Knockdown of lncRNA ENST00000609755.1 Confers Protection Against Early oxLDL-Induced Coronary Heart Disease

Background: This study investigated the association between long non-coding RNAs (lncRNAs) and coronary heart disease (CHD) further elucidated potential biological roles of lncRNAs in CHD pathogenesis. Methods: A case-control (590 patients 590 controls) was conducted from February 2017 March 2019 Fuzhou, China. Environmental factors were using questionnaires physical examinations. Five representative screened lncRNA microarray (peripheral blood 5 cases verified by quantitative real-time polymerase chain reaction leukocyte 100 controls). Oxidized low-density lipoprotein (oxLDL) used to induce a human artery endothelial cell (HCAECs) injury model, loss function elucidate role ENST00000609755.1 (lnc-MICALL2-2) oxLDL-induced HCAECs injury. Results: total 320 found dysregulated (fold change> 2, p < 0.05). The results discovery microarray, population verification HCAEC experiments suggested lnc-MICALL2-2 is upregulated subjects an model. Conversely, inhibition vitro attenuated effects oxLDL on morphology, proliferation, apoptosis. Conclusion: Elevated expression independent risk factor for CHD, knockdown subsequently confers protection against early pathological processes CHD.