Knockdown of cathepsin D in zebrafish fertilized eggs determines congenital myopathy
نویسندگان
چکیده
منابع مشابه
Knockdown of cathepsin D in zebrafish fertilized eggs determines congenital myopathy
CD (cathepsin D) is a ubiquitous lysosomal hydrolase involved in a variety of pathophysiological functions, including protein turnover, activation of pro-hormones, cell death and embryo development. CD-mediated proteolysis plays a pivotal role in tissue and organ homoeostasis. Altered expression and compartmentalization of CD have been observed in diseased muscle fibres. Whether CD is actively ...
متن کاملKnockdown of fast skeletal myosin-binding protein C in zebrafish results in a severe skeletal myopathy
Myosin-binding protein C (MyBPC) in the muscle sarcomere interacts with several contractile and structural proteins. Mutations in the cardiac isoform (MyBPC-3) in humans, or animal knockout, are associated with cardiomyopathy. Function of the fast skeletal isoform (MyBPC-2) in living muscles is less understood. This question was addressed using zebrafish models, combining gene expression data w...
متن کاملPronuclear Microinjection of Rabbit Fertilized Eggs
Although, the first transgenic animal was produced more than 30 years ago, there are still many factors limiting efficiency of transgenesis. One of them is the low rate of transgene incorporation into the genome of microinjected eggs. This mini-review summarizes recent research methods based on pronuclear microinjection of rabbit fertilized eggs in order to reach higher efficiency of transgene ...
متن کاملElectrophoretic movement of fertilized sea-urchin eggs.
The eccentric shift of the sea-urchin egg within the fertilization membrane under an electric field was analysed by measuring the electrophoretic mobility of the isolated fertilization membrane and that of the egg deprived of the fertilization membrane. In addition, the migration speed of the egg proper was measured within the fertilization membrane under the conditions that: either (1) the mov...
متن کاملCathepsin D deficiency underlies congenital human neuronal ceroid-lipofuscinosis.
Congenital neuronal ceroid-lipofuscinosis (NCL) is a devastating inherited neurodegenerative disorder of unknown metabolic basis. Eight patients with this rare disorder, all with similar clinical and neuropathological findings, have been reported, and here we describe two further patients. Previously, we showed that a mutation in the cathepsin D gene causes congenital NCL in sheep. On the basis...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioscience Reports
سال: 2013
ISSN: 0144-8463,1573-4935
DOI: 10.1042/bsr20120100