Kinetic studies on recombinant stem bromelain

Fractionation and specificity studies on stem bromelain.

The presence, in the juice of pineapples, of a proteolytic enzyme or enzymes under the name of bromelin (2) has long been known, and some of the characteristics of this enzyme have been described (3-7). The ,juice of the stem of the pineapple plant was found by Heinicke and Gortner (8) to contain a similar enzyme. The acetone precipitate from the stem juice, which is called stem bromelain,’ is ...

Hydrolysis of bradykinin by stem bromelain.

During the course of studies on the anti-edema and anti-inflammatory actions of stem bromelain, a potent kininase activity was observed (1). The present paper describes the analysis of the bonds of bradykinin which are susceptible to the action of stem bromelain. Minoh, Osaka) dissolved in 3 ml of 5 mM phosphate buffer, pH 7.5, containing 2 mM (3 mercaptoethanol, was mixed with 3 ml of purified...

Recovery of recombinant bromelain from Escherichia coli BL21-AI

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombina...

Kinetic studies on sorbose fermentation.

Kinetic studies on rabbitmuscle myokinase.