Kinetic studies of Escherichia coli AlkB using a new fluorescence-based assay for DNA demethylation
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Kinetic studies of Escherichia coli AlkB using a new fluorescence-based assay for DNA demethylation
The Escherichia coli AlkB protein catalyzes the direct reversal of alkylation damage to DNA; primarily 1-methyladenine (1mA) and 3-methylcytosine (3mC) lesions created by endogenous or environmental alkylating agents. AlkB is a member of the non-heme iron (II) alpha-ketoglutarate-dependent dioxygenase superfamily, which removes the alkyl group through oxidation eliminating a methyl group as for...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkm1031