Kinetic mechanism of dihydropyrimidine dehydrogenase from pig liver.
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چکیده
منابع مشابه
Kinetic mechanism of chicken liver xanthine dehydrogenase.
The kinetic behaviour of chicken-liver xanthine dehydrogenase (xanthine/NAD+ oxidoreductase; EC 1.2.1.37) has been studied. Steady-state results, obtained from a wide range of concentrations of substrates and products, were fitted by rational functions of degree 1:1, 1:2, 2:2 and 3:3 with respect to substrates, and 0:1, 1:1, 0:2 and 1:2 with regard to products, using a non-linear regression pro...
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Although dihydropyrimidine dehydrogenase has been purified to varying degrees from several species, very little is known about the human enzyme. The importance of this enzyme has recently been shown with cancer chemotherapy, particularly in patients with genetic deficiency of this enzyme. In the present study, this enzyme was purified 7800-fold to homogeneity from human liver by introducing sev...
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The kinetic mechanism of lipoamide dehydrogenase has been studied at pH 8.0, 37”, using the enzyme from rat liver mitochondria. Initial velocity patterns obtained for both the forward and reverse reactions were a series of parallel lines. Michaelis constants for the reactants are: NAD, 0.52 mu; dihydrolipoamide, 0.49 mu; NADH, 0.062 mM; lipoamide, 0.84 mu. Isotopic exchange between NAD and NADH...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)38254-7