Kinetic analysis of ribonucleic acid chain initiation by Escherichia coli Ribonucleic acid polymerase bound to DNA

Kinetic analysis of ribonucleic acid chain initiation by Escherichia coli Ribonucleic acid polymerase bound to DNA.

The kinetics of the RNA chain initiation reaction carried out by RNA polymerase bound to the initiator region of a DNA template have been analyzed. Initiation proceeds in a two-substrate reaction in which the initial binary complex (enzyme-DNA) is transformed into a ternary complex (enzyme-DNA-RNA) by formation of a dinucleoside tetraphosphate and release of inorganic pyrophosphate. In this rea...

Membrane-bound ribonucleic acid synthesis in Escherichia coli infected with ribonucleic acid bacteriophage R23.

1. A cell membrane-DNA-RNA polymerase complex which contains virtually all of the RNA-synthetic capacity of the cell has been isolated from uninfected and R23-infected Escherichia coli. 2. Infection of E. coli by RNA bacteriophage R23 inhibits the RNA synthetic capacity of the membrane complex. In infected cells some of the RNA synthetic capacity is released from the membrane fraction. 3. The m...

Binding sites on Escherichia coli ribonucleic acid polymerase.

Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase.

The fidelity of in vitro transcription of bacteriophage T3 DNA by T3 RNA polymerase and Escherichia coZi RNA POlymerase have been studied by DNA-RNA hybridizationcompetition studies with regard to (a) asymmetry of transcription and (b) the region of the T3 DNA transcribed by either polymerase. RNA transcribed by T3 RNA polymerase hybridized exclusively with the H strand of T3 DNA-the only stran...

Regulation of ribonucleic acid synthesis in Escherichia coli during diauxie lag: accumulation of heterogeneous ribonucleic acid.

The synthesis of ribonucleic acid (RNA) and of protein in Escherichia coli during glucose-lactose diauxie lag have been examined. The rate of RNA synthesis is about 7%, of the corresponding rate during exponential growth and the rate of protein synthesis 10 to 15%. Inhibition of RNA synthesis occurs to the same extent in both rel and rel(+) strains. The RNA which accumulates during 20 min in di...