Kinase Assay Based on Thiophosphorylation and Biotinylation
نویسندگان
چکیده
منابع مشابه
Kinase assay based on thiophosphorylation and biotinylation.
Protein kinases catalyze the transfer of the gamma-phosphate group from ATP to a serine, threonine or tyrosine residue of an acceptor protein. These enzymes play an important role in signal transduction. New inhibitors for these enzymes are actively being sought. In this article, we present a novel approach for detecting the activity of protein kinases, which could be useful for the high-throug...
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Specificity and localization of the acetyicholine receptor kinase ( membrane protein phosphorylation / hypotonic lysis / detergents / thiophosphorylation )
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99276rr01