منابع مشابه
Labeling of Human Serum Albumin with Stable Isotope of Bromine; an in Vitro Study
Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...
متن کاملStable Isotope Labeling in Proteomics
Proteomics, the analysis of the proteins expressed by a cell, tissue or organism under a specific set of conditions, has undergone a tremendous period of growth in the past few years. Proteomic studies are typically designed to analyze hundreds or thousands of proteins in a single analysis and aim to provide a global view of changes in protein expression that occur in different cellular growth ...
متن کاملStable Isotope Labeling of Mammals (SILAM).
INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., (2)H, (13)C, (15)N, or (18)O). Introduction of stable isotope labels into proteins alters their molecular weight and such changes can be observed on the mass spectrome...
متن کاملIsotope-coded, fluorous photoaffinity labeling reagents.
A pair of isotope-coded, fluorous photoaffinity labeling reagents has been developed and coupled with a peptide. The modified peptides form adducts with methanol upon light illumination, which show characteristic isotope labeling patterns in mass spectra and can be separated from other peptides through fluorous silica.
متن کاملIsotope Labeling Methods for Protein Dynamics Studies
Protein structure determination by solution NMR spectroscopy has long relied on the uniform stable isotopic enrichment with 13C and 15N to alleviate resonance overlap and to allow multiple distance and angular restraints, at as many atomic sites as possible, to facilitate computing the optimal three-dimensional structural model.(1) Recently, the optimization of these labeling techniques has inc...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1965
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2088