Isothermal Titration Calorimetry in Biocatalysis

Isothermal titration calorimetry (ITC) is a popular chemical analysis technique that can be used to measure macromolecular interactions and physical processes. ITC involves the measurement of heat flow from cell after each injection during experiment. has been useful thermodynamics such as protein-ligand or protein-protein binding affinity also processes enzyme catalyzed reactions. The use in biocatalysis number advantages enables kinetic parameters direct manner and, principle, for most enzymes substrates. approaches have developed reversible irreversible inhibition, effects molecular crowding on activity, activity immobilized conversion complex polymeric A disadvantage order obtain accurate special care taken proper experimental design data interpretation, which unfortunately not always case reported studies. Furthermore, caution necessary when experiments are performed include solvents, reducing agents may side An important bottleneck relatively low throughput, solved future by sensitive chip based microfluidic calorimetric devices.