Isolation of transforming DNA by cosmid rescue.
نویسندگان
چکیده
منابع مشابه
Inhibition of Hepatocyte DNA Synthesis by Transforming
Previous studies have shown that both transforming growth factor 0 (TGF-/3) and cyclic AMP (cAMP) inhibit hepatocyte DNA synthesis. While cAMP (in addition to being stimulatory in Go/early GO exerts its inhibition on hepatocytes late in (.,. the point where TGF-/3 inhibits has not been precisely defined. We have now examined further the inhibitory effects of cAMP and TGF-/3, on DNA synthesis in...
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A method has been developed that allows the isolation of genomic clones from a cosmid library by homologous recombination in vivo. This method was used to isolate a human genomic interleukin 2 (IL2) gene. The genomic cosmid library was packaged in vivo into lambda phage particles. A recombination-proficient host strain carrying IL2 cDNA sequences in a non-homologous plasmid vector was infected ...
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BACKGROUND The formation of DNA knots is common during biological transactions. Yet, functional implications of knotted DNA are not fully understood. Moreover, potential applications of DNA molecules condensed by means of knotting remain to be explored. A convenient method to produce abundant highly knotted DNA would be highly valuable for these studies. RESULTS We had previously shown that c...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1982
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.79.2.520