ISOLATION OF THREE MAIN COMPONENTS, F3, F4 AND F5, FROM AZALOMYCIN F-COMPLEX

Isolation of three main components. F3, F4 and F5, from azalomycin F-complex.

Isolation of azalomycins F3, F4 and Fg from azalomycin F-complex crystals was successfully conducted. F3 was less soluble in methanol than other components and was isolated by fractional crystallization of F-complex from 80~ 100% methanol in water. F4 and F5 were separated by P-cellulose column chromatography, from which F5 and F4 were eluted with 60 % aqueous methanol and 0.01M ammonium acetat...

Demalonyl derivatives of azalomycin F4 and scopafungin.

Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5

Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the b...

Classification of Self-Orthogonal Codes over F3 and F4

Several methods for classifying self-orthogonal codes up to equivalence are presented. These methods are used to classify self-orthogonal codes with largest possible minimum distance over the fields F3 and F4 for lengths n ≤ 29 and small dimensions (up to 6). Some properties of the classified codes are also presented. In particular, an extensive collection of quantum error-correcting codes is o...

Effect of azalomycin F on bacteria.

It has previously been reported that azalomycin F could induce permeability changes in Candida albicans causing loss of cell constituents and inhibition of substrate uptake which resulted in the death of the organism1). The present report is concerned with the effect of the antibiotic on bacteria. Methods and Results Azalomycin F inhibited the growth of Bacillus subtilis PCI 219 at concentratio...