Isolation of Substance P Binding Protein from Rat Brain
نویسندگان
چکیده
منابع مشابه
Ontogeny of substance P receptor binding sites in rat brain.
The ontogeny of substance P (SP) receptor binding sites in rat brain has been studied using both membrane binding assays and in vitro receptor autoradiography. The density of SP binding sites is maximal 1 d before birth and decreases thereafter to reach adult values 14 d after birth. During the early postnatal period, the distribution of SP binding sites undergoes major modifications. For examp...
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The characterization and subsequent availability of synthetic substance P has renewed interest in the role of this undecapeptide in central-nervous-system function (Mroz & Leeman, 1974). Biochemical and electrophysiological studies have demonstrated that substance P may function as an excitatory transmitter in primary sensory neurons terminating in the spinal cord (Otsuka & Konishi, 1975). In t...
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In aqueous extracts of rat brain, spinal cord, and sciatic nerve, a protein antigen has been detected which could not be shown in similarly prepared extracts of a variety of other rat tissues. The protein, designated antigen (Y, had a high mobility in zone electrophoresis at pH 7.4. Antigen cr was isolated and was found to consist of a single type of subunit having a molecular weight of about 3...
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Most synapses in the central nervous system exhibit a prominent electron-opaque specialization of the postsynaptic plasma membrane called the postsynaptic density (PSD). We have developed a procedure for the isolation of PSDs which is based on their buoyant density and their insolubility in N-lauroyl sarcosinate. Treatment of synaptic membranes with this detergent solubilizes most plasma membra...
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Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific bin...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1992
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)37628-0