Isolation of Rat Transferrin Using CNBr-Activated Sepharose 4B

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Isolation of rat transferrin using CNBr-activated sepharose 4B.

1. The isolation of transferrin from rat serum by means of affinity chromatography on CNBr-activated Sepharose 4 B is described. -2. Subfractionation by isoelectric focusing yielded two transferrin fractions with identical biological behaviour but with small differences in isoelectric point (6.0 and 5.8) and sialic acid contents.

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Preparation of factor VII concentrate using CNBr-activated Sepharose 4B immunoaffinity chromatography

BACKGROUND Factor VII concentrates are used in patients with congenital or acquired factor VII deficiency or treatment of hemophilia patients with inhibitors. In this research, immunoaffinity chromatography was used to purify factor VII from prothrombin complex (Prothrombin- Proconvertin-Stuart Factor-Antihemophilic Factor B or PPSB) which contains coagulation factors II, VII, IX and X. The aim...

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CNBr-activated Sepharose 4 Fast Flow

Ligands are coupled to CNBr-activated Sepharose 4 Fast Flow using a rapid and efficient process. The coupled ligand maintains a high level of biological activity. • Rapid, efficient coupling and maintained biological activity of the ligand • Multi-point attachment of many protein ligands, resulting in a chemically stable product. • Fast Flow matrix gives high productivity and is easy to scale u...

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Preparation of factor VII concentrate using CNBr-activated

  Background: Factor VII concentrates are used in patients with congenital or acquired factor VII deficiency or treatment of hemophilia patients with inhibitors. In this research, immunoaffinity chromatography was used to purify factor VII from prothrombin complex (Prothrombin-Proconvertin-Stuart Factor-Antihemophilic Factor B or PPSB) which contains coagulation factors II, VII, IX and X. The a...

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Preparation of factor VII concentrate using CNBr-activated

Background: Factor VII concentrates are used in patients with congenital or acquired factor VII deficiency or treatment of hemophilia patients with inhibitors. In this research, immunoaffinity chromatography was used to purify factor VII from prothrombin complex (ProthrombinProconvertin-Stuart Factor-Antihemophilic Factor B or PPSB) which contains coagulation factors II, VII, IX and X. The aim ...

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ژورنال

عنوان ژورنال: Clinical Chemistry and Laboratory Medicine

سال: 1976

ISSN: 1434-6621,1437-4331

DOI: 10.1515/cclm.1976.14.1-12.475