Isolation of motile spermatozoa from semen samples using microfluidics

Sperm tail flexibility test: a simple test for selecting viable spermatozoa for intracytoplasmic sperm injection from semen samples without motile spermatozoa.

PURPOSE The objective was to describe the results of the injection of immotile spermatozoa with flexible tails when only immotile spermatozoa are present in the semen sample. METHODS A retrospective study was conducted to analyze the procedure results for 10 couples who participated in our intracytoplasmic sperm injection program. The sperm tail was considered flexible when it moved up and do...

Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats

Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease ingoats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semensamples were collected from goat bulks, and all samples were cultured ...

The Influence of Sperm Morphology, Total Motile Sperm Count of Semen and the Number of Motile Sperm Inseminated in Sperm Samples on the Success of Intrauterine Insemination

Zika virus isolation from semen.

Zika virus (ZIKV) can be sexually transmitted and replicative particles were first detected in a semen sample from a patient during the 2013-14 French Polynesia outbreak. Here we describe the virus isolation from semen of a patient returning to Italy from Brazil.

Isolation of Legionella pneumophila from Tehran Hospital Water Samples

Over a Period of three months (summer), 108 samples of water collected from four hospitals in Te-hran. They were taken from numerous potable and non-potable water, including main supply cis-terns, calorifiers, hot and cold tap water, shower, cooling tower and drain of air condition system. Culturing of concentrated and non-concentrated water samples on Buffered Charcoal Yeast Extract (BCYE) Aga...