Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
نویسندگان
چکیده
منابع مشابه
Isolation of human hepatocytes by a two-step collagenase perfusion procedure.
The liver, an organ with an exceptional regeneration capacity, carries out a wide range of functions, such as detoxification, metabolism and homeostasis. As such, hepatocytes are an important model for a large variety of research questions. In particular, the use of human hepatocytes is especially important in the fields of pharmacokinetics, toxicology, liver regeneration and translational rese...
متن کاملStructural studies of human IgD: isolation by a two-step purification procedure and characterization by chemical and enzymatic fragmentation.
A myeloma IgD immunoglobulin (designated WAH) that was present in high concentration in plasma ( approximately 3.5 g/dl) was purified in >90% yield by a two-step procedure of ammonium sulfate precipitation plus AcA 34 gel filtration. Although the plasma had been stored for 2 years without the addition of a proteolytic inhibitor, no "spontaneous" degradation was apparent and the isolated IgD rem...
متن کاملSuccessful human islet isolation utilizing recombinant collagenase.
The enzymatic dissociation of acinar tissue by collagenase is a substantial step in the isolation of pancreatic islets. Although essential collagenase components have been purified, the variability in the activity of different batches limits long-term reproducibility of isolation success. The utilization of purified recombinant proteases would solve this problem. In the present study, pancrease...
متن کاملDigitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes.
Intact rat liver cells from the perivenous region were isolated by collagenase perfusion after first destroying the periportal region by a brief portal infusion of digitonin. Periportal cells were isolated after retrograde digitonin infusion. Significantly higher alanine aminotransferase, gamma-glutamyltransferase and lactate dehydrogenase activities and lower glutamate dehydrogenase and pyruva...
متن کاملInvestigation the Consumer Choice in the Use of Dental Services Care Applying Hekman Two-Step Procedure
Considering the health importance in development process of countries, this study investigates factors affecting various types of dental care services participation and related expenditures as one of the most important aspects of oral health. For this reason, a sample of almost 40000 Iranian households in 2016 is selected and the impact of the mentioned factors analyzed using Heckman’s two-stag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/50615