Isolation of Cytosol, Microsome, Free Polysomes (FPs) and Membrane-bound Polysomes (MBPs) from Arabidopsis Seedlings
نویسندگان
چکیده
منابع مشابه
Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
Small RNAs are central players in RNA silencing, yet their cytoplasmic compartmentalization and the effects it may have on their activities have not been studied at the genomic scale. Here we report that Arabidopsis microRNAs (miRNAs) and small interfering RNAs (siRNAs) are distinctly partitioned between the endoplasmic reticulum (ER) and cytosol. All miRNAs are associated with membrane-bound p...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2017
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2436