Isolation of Clara cells from the mouse lung.
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چکیده
منابع مشابه
Isolation of Clara cells from the mouse lung.
A method is described for isolating Clara cells from the mouse lung that does not require the technique of elutriation. Mouse lungs totally perfused of blood are instilled with crystalline trypsin (0.25%) and incubated for the optimum time of 15 min. The lung tissue is chopped, mechanically agitated, and sequentially filtered to obtain a primary digest of 3 to 5 x 10(6) cells. Clara cells, iden...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1990
ISSN: 0091-6765,1552-9924
DOI: 10.1289/ehp.85-1568317