Isolation of Chlamydia using McCoy cells and Buffalo green monkey cells.
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چکیده
منابع مشابه
Isolation of Chlamydia using McCoy cells and Buffalo green monkey cells.
Unselected eye and genital specimens from 233 patients were cultured for Chlamydia. The isolation rates were compared using McCoy cells and Buffalo green monkey cells, both procedures being performed with and without the addition of cycloheximide and centrifugation. No significant difference was found in the isolation rates using the four methods. The characteristics of the two cell lines and t...
متن کاملComparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.
A total of 1,229 urogenital samples of patients with sexually transmitted diseases and prostitutes were inoculated simultaneously in McCoy cells and Buffalo green monkey (BGM) cells by using a microtitration technique. BGM cells proved to be slightly more sensitive than McCoy cells, and they usually showed more and larger inclusions and were more resistant to cytotoxic substances in samples. Th...
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Specimens from eye and genital tract were cultured in parallel in irradiated and non-irradiated McCoy cells and the frequency of isolation of chlamydia using these culture methods was compared. There was a significant difference between the frequencies of isolation; irradiated McCoy cells produced a greater number of positive results.
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Resveratrol (RESV), an antifungal compound from grapes and other plants, has a distinct ability to inhibit the Chlamydia (C.) trachomatis developmental cycle in McCoy cells, a classic cell line used for chlamydial research. Inoculation of C. trachomatis with increasing amounts of RESV (from 12.5 to 100 μM) gave a dose-dependent reduction in the number of infected McCoy cells visualized by using...
متن کاملCultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.
An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metaboli...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1984
ISSN: 0021-9746
DOI: 10.1136/jcp.37.2.120