Isolation of Campylobacter spp. from stool specimens with a semisolid medium.
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چکیده
منابع مشابه
Semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.
Isolation of Campylobacter jejuni and C. coli from stool specimens is done by growing campylobacter colonies on solid selective media with or without blood. However, recognition of these colonies can be difficult. Therefore, we decided to evaluate an isolation procedure based on the swarming of campylobacters through a semisolid medium. We developed a semisolid blood-free selective motility (SS...
متن کاملComparison of six media, including a semisolid agar, for the isolation of various Campylobacter species from stool specimens.
A recently described semisolid blood-free selective motility medium (SSM) (J. Goossens, L. Vlaes, I. Galand, C. Van den Borre, and J. P. Butzler, J. Clin. Microbiol. 27:1077-1080, 1989) was compared with two charcoal-based selective media (charcoal-based selective medium [CSM] and modified charcoal cefoperazone deoxycholate agar [CCDA]), two blood-based media (Skirrow medium [SKM] and CampyBAP)...
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In 2010, we surveyed 176 clinical laboratories in Pennsylvania regarding stool specimen testing practices for enteropathogens, including Campylobacter spp. Most (96.3%) routinely test for Campylobacter spp. In 17 (15.7%), a stool antigen test is the sole method for diagnosis. We recommend that laboratory practice guidelines for Campylobacter spp. testing be developed.
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The Cult-a-Box gas generator tablet method for isolating Campylobacter spp. from stool cultures was evaluated by comparison with a standard gas mixture of 5% oxygen, 10% carbon dioxide, and 85% nitrogen. Of the 186 positive cultures, 90.3% were found by both methods. Of the remaining 9.7% which were positive in one system only, 11 were found by the tablet method and 7 by the standard gas method.
متن کاملComparison of Salmonella chromogenic medium with DCLS agar for isolation of Salmonella species from stool specimens.
Salmonella chromogenic medium (SCM; Oxoid, Basingstoke, United Kingdom), a new selective chromogenic medium, was compared to DCLS agar (Oxoid) for the detection and presumptive identification of Salmonella species from stool samples. This medium contains two chromogenic substrates, Magenta-cap (5-bromo-6-chloro-3-indolylcaprylate), which is hydrolyzed by Salmonella species to give magenta colon...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.11.2681-2682.1991