Isolation of a cDNA Encoding Cytosolic Ascorbate Peroxidase in Tobacco
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چکیده
منابع مشابه
Isolation and characterization of a cDNA for spinach cytosolic ascorbate peroxidase.
Reduced oxygen intermediates are produced in plant tissues from mitochondrial and chloroplastic electron transport pathways and from electron-saturated photosynthetic pigments (Bowler et al., 1992). For instance, the Mehler reaction is a primary source of reduced oxygen intermediates in chloroplasts in which dioxygen is reduced by Fd on the reducing side of PSI to produce .O2-. The dismutation ...
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As a means to eliminate pathogen-infected cells and prevent diseases, programmed cell death (PCD) appears to be a defense strategy employed by most multicellular organisms. Recent studies have indicated that reactive oxygen species, such as O2.- and H2O2, play a central role in the activation and propagation of pathogen-induced PCD in plants. However, plants contain several mechanisms that deto...
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Switchgrass (Panicum virgatum L.) is being developed as a bioenergy species. Recently an early version of its genome has been released permitting a route to the cloning and analysis of key proteins. Ascorbate peroxidases (APx) are an important part of the antioxidant defense system of plant cells and present a well studied model to understand structure-function relationships. Analysis of the ge...
متن کاملCharacterization and crystallization of recombinant pea cytosolic ascorbate peroxidase.
An Escherichia coli expression system has been developed for pea cytosolic ascorbate peroxidase (APX). The enzyme was expressed as a fusion product with the E. coli maltose-binding protein for rapid, affinity chromatography purification. Recombinant ascorbate peroxidase (rAPX) was purified by tryptic digestion to separate the maltose-binding protein from rAPX followed by three chromatographic s...
متن کاملAcclimation to diverse environmental stresses caused by a suppression of cytosolic ascorbate peroxidase in tobacco BY-2 cells.
The active oxygen species (AOS) that arise from normal metabolic processes are kept under tight control by various antioxidant mechanisms. AOS are important signal molecules that regulate many physiological processes, including environmental stress responses. In this work, we have investigated the effect of lowered cytosolic ascorbate peroxidase (APX) activity in transgenic tobacco BY-2 cells, ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1995
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.108.2.839