Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis

Fibrinolytic therapy progressed by the evolution of different strategies that helped in enhancing its efficacy and specificity. The use microbial fibrinolytic enzymes is leading to a promising direction for treatment cardiovascular diseases. Subtilisin, member subtilases enzyme abundantly found Bacillus species. isolation subtilisin gene (quaking homolog) from subtilis was attempted present work. genomic deoxyribonucleic acid extraction done following Yamada protocol used as template polymerase chain reaction amplification target using specifically designed primers. product ligated into cloning vector pTZ57R/T followed transformation Escherichia coli top 10 strain. A 1090 base pair, partial sequence amplified coding protein 363 amino acids with molecular weight 37550.7 Daltons. nucleotide revealed significant evolutionary relationships other strains subtilis. Our study confirms presence local species suggesting economical way produce important thrombolytic agents commercial pharmaceutical worth.